1. HOME
  2. The production of biopharmaceuticals by transgenic chicken

The production of biopharmaceuticals by transgenic chicken

  • School of Engineering/Graduate School of Engineering

Shinji iijima [Professor]

http://www.nubio.nagoya-u.ac.jp/nubio3/index.htm

Outline of Seeds

The chickens and other transgenic animals could replace current bioreactor process in the low-cost production of therapeutic antibodies and other biopharmaceuticals as a next-generation technology.
By introducing antibody DNA into chicken embryos followed by artificial hatching, we have succeeded in creating transgenic chickens. Experiments conducted using model antibodies have resulted in the production in egg white of 2-100 milligrams of the antibody per egg.

Novelty and originality of this research

Several research groups have already succeeded in advanced research on establishing transgenic cows and sheep, but the development of transgenic technology in chickens (which are smaller and easier to keep) still lags behind. By adjusting the timing of genetic manipulation, we have been able to develop transgenic chicken that produced useful substances at high levels.

Application and research area for Industry collaboration

Production of useful proteins and other biopharmaceuticals, more efficient vaccine production, production of influenza vaccines

Key Takeaway

Aiming to speed up basic and applied research on chromosomes, carbohydrate chains, and similar compounds

Keywords

Transgenic chicken, retrovirus, substance production, carbohydrate complex containing sialic acid, glycotechnology

Technologies

  • DNA analysis
  • Transgenic chicken production

Equipment

  • Quantitative PCR
  • Confocal laser microscopy
  • Biacore
  • Cell sorter

Monographs, Papers and Articles

  • High-level expression of single-chain Fv-Fc fusion protein in serum and egg white of genetically manipulated chickens using a retroviral vector, Journal of Virology, 79[17], 10864-10874 (2005)
  • Production of recombinant tumor necrosis factor receptor/Fc fusion protein by genetically manipulated chickens, Journal of Bioscience and Bioengineering, 105{5}, 454-459 (2008)
  • Production of chimeric monoclonal antibodies by genetically manipulated chickens, Journal of Biotechnology, 141, 18-25 (2009)
  • Production of transgenic chickens from purified primordial germ cells infected with a lentiviral vector, Journal of Bioscience and Bioengineering, 109(4), 315-321 (2010)
  • Chicken oviduct-specific expression of transgene by a hybrid ovalbumin enhancer and the Tet expression system, Journal of Bioscience and Bioengineering, 113(2), 146–153 (2012)
  • Genetic modification of a chicken expression system for the galactosylation of therapeutic proteins produced in egg white, Transgenic Research, 21(1), 63-75 (2012)
  • Transgenic Chicken , Development Growth and Differentiation, 55, 207-216(2013)
  • Recombinant proteins produced into yolk of genetically manipulated chickens are partly sialylated in N-glycan. Cytotechnology (2013) in press
  • Galactosylation of erythropoietin produced by a transgenic chicken expressing galactosyltransferase. Journal of Bioscience and Biotechnology. 117, 676-679(2014)