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Regulation of inflammation by lectin

  • School of Engineering/Graduate School of Engineering
  • Laboratory of Genetic Engineering and Molecular Biology
  • Department of Biotechnology

Kenichi Nishijima [Associate Professor]

Outline of Seeds

Medical importance of carbohydrate chains based on their many physiological functions including immune regulation. We focused on the relationship between carbohydrate chains and stimulation/inhibition of immune responses. In particular, we found that a kind of carbohydrate chain binding protein existed on the surface of leukocyte or lymphocyte inhibited inflammatory cytokine production and increased anti- inflammatory cytokine production. We mainly studied this mechanism and applications to medicine.

Novelty and originality of this research

We found a kind of carbohydrate chain binding protein (lectin) inhibited inflammation.

Application and research area for Industry collaboration

Development of anti-inflammatory drugs based on new principles.

Key Takeaway

We aim to rapidly move from basic study to practical study, for example, in studies of chromosome, immunity, or carbohydrate chain.

Keywords

Carbohydrate chain containing sialic acid, innate immunity, bacteria infection, activation of immunity, inflammation, cytokine, lectin

Technologies

  • DNA analysis
  • Assay of activator/inhibitor of immunity
  • Complex carbohydrate chain analysis
  • Antibody production
  • Chimeric antibody

Equipment

  • Quantitative PCR
  • Confocal laser scanning microscope
  • Cell sorter

Monographs, Papers and Articles

  • Siglec-9 enhances IL-10 production in macrophages via tyrosine-based motifs, Biochemical and Biophysical Research Communications, 369, 878-883 (2008)
  • Effects of Siglec on the expression of IL-10 in the macrophage cell line RAW264. Cytotechnology, DOI 10.1007/s10616-014-9717-0 (2014)
  • Enhanced lentiviral vector production in 293FT cells expressing Siglec-9. Cytotechnology, DOI 10.1007/s10616-013-9679-7 (2014)
  • Lectin-dependent localization of cell surface sialic acid-binding lectin Siglec-9. Cytotechnology, DOI 10.1007/s10616-014-9691-6 (2014)