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Newly developed analytical technology to achieve narrow sampling and infinitesimal sample amount

  • School of Medicine/Graduate School of Medicine
  • Comprehensive Medicine
  • Social Life Sciences
  • Forensics Medicine, Bioethics
  • (Nagoya University's Institute for Advanced Research - Young researcher units for the advancement of new and undeveloped fields - In vivo real-time omics unit)

Kei Zaitsu [Associate Professor]

  • School of Medicine/Graduate School of Medicine
  • Radiological and Medical Laboratory Sciences
  • Pathophysiological Laboratory Sciences
  • (Nagoya University's Institute for Advanced Research - Young researcher units for the advancement of new and undeveloped fields - In vivo real-time omics unit)

Yumi Hayashi [Assistant Professor]

Outline of Seeds

Probe electrospray ionization (PESI) is an ionization method first developed by Dr. Kenzo Hiraoka at the University of Yamanashi. Because the method uses an extremely fine-tipped needle (probe) with a diameter of just 700 nm for sampling and ionization, it makes it possible to conduct analysis without pretreatment (e.g. extraction operations) on trace amounts of just a few pL for easy, rapid analysis. Were working with Shimadzu Corporation to combine this PESI method with tandem mass spectrometry (MS/MS) and its advanced compound identification capability in order to develop a method that enables high-sensitivity analysis using trace amounts and ultra-narrow fields. The use of our method makes it possible to conduct direct analysis without pretreating serum or other samples drawn from living tissue or metabolic products (metabolites) like amino acids. We have also taken advantage of the low invasiveness of the probe in order to develop a method for analyzing metabolites directly and in real time using live mouse organs. Promising applications include real-time analysis, trace amount analysis, and minimally invasive analysis.

Novelty and originality of this research

By combining the innovative ionization method PESI with the advanced compound identification ability of tandem mass spectrometry, we are leading the world in putting together tools for the intact analysis of intraorgan metabolites in real time. Because our method makes it possible to conduct direct analysis of metabolites inside organs and rapidly analyze drugs in the blood, it is well-suited to applications involving rapid/local/real-time analysis of low molecular weight compounds.

Application and research area for Industry collaboration

Because our method allows for rapid low molecular analysis, analysis of trace amounts, real-time monitoring, and a wide variety of other applications for target compounds, it has the potential for applications in paramedic or poisoning analysis, for example, if drugs are targeted. If metabolites like amino acids or organic acids are targeted, it could be used in clinical applications as well, such as rapid analysis of biomarkers. Further application ideas include quality management for food products or on-site inspections of chemical materials.

Keywords

PESI, tandem mass spectrometry, real-time analysis, non-invasive sampling

Equipment

  • Probe electrospray ionization (PESI) - tandem mass spectrometry (MS/MS)

Monographs, Papers and Articles

  • K Zaitsu, Y Hayashi, et al. Analytical Chemistry, 2016, 88 (7), pp 3556‐3561