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Cell-selective peptides for regeneration-enhancing medical devices

  • Graduate School of Pharmaceutical Sciences
  • Department of Basic Medicinal Sciences
  • Laboratory of Cell and Molecular Bioengineering
  • Division of Biosciences

Kei Kanie [Assistant Professor]

http://www.ps.nagoya-u.ac.jp/lab_pages/CMB/index-e.html

  • Graduate School of Pharmaceutical Sciences
  • Department of Basic Medicinal Sciences
  • Laboratory of Cell and Molecular Bioengineering
  • Division of Biosciences

Ryuji Kato [Associate Professor]

Outline of Seeds

For medical devices that are implanted for long time in vivo, their surface functionalization to proper interact with various types of cells is important for reducing side effects, and also important for enhancing their in vivo regeneration. Our seed technology is "short peptides (3AA to 9AA)" for providing cell-selective performance on solid phase surfaces. These peptides can attract objective cells for regeneration, but can relatively inhibit the adhesion of unwanted cells, which we designate them as "cell-selective peptides". We have more than 30 types of candidate sequences to control the adhesion of endothelial cells, smooth muscle cells, and fibroblasts.

Novelty and originality of this research

In most of the cell adhesion peptides, their function is weighted on "strong adhesion", therefore "elimination of unwanted cells" are not considered. Our peptides are screened from the combinatorial peptide array library by multiple assays of various cells, therefore have the performance of "attracting objective cells, but inhibiting unwanted cells". Our peptide candidates are also very short, such as 3-mer. This enables to produce such material with low cost and high purity, which is important for medical material production.

Application and research area for Industry collaboration

We have been applying this technology for various applications;
- Artificial vessels (small diameter)
- Inhibition of tissue adhesion
We are welcome to make collaborations with companies that produce medical devices, or produce medical polymers.

Key Takeaway

We have not only the peptide sequences, but also the screening technology for such cell-selective peptides, therefore can make application to wider objectives.

Keywords

Medical devices, Cell adhesion, Cell selectivity, Peptides, Peptide screening, Peptide coating

Technologies

  • Cell-selective peptide sequences
  • Peptide array-based peptide screening method
  • Cell-selective peptide coating know-how

Monographs, Papers and Articles

  • Kanie K, Narita Y, Zhao Y, Kuwabara F, Satake M, Honda S, Kaneko H, YoshiokaT, Okochi M, Honda H, Kato R. Collagen type IV-specific tripeptides for selectiveadhesion of endothelial and smooth muscle cells. Biotechnol Bioeng. 2012, 109(7), 1808-16
  • Kanie K, Kato R, Zhao Y, Narita Y, Okochi M, Honda H. Amino acid sequencepreferences to control cell-specific organization of endothelial cells, smoothmuscle cells, and fibroblasts. J Pept Sci. 2011, 17(6), 479-86.
  • Kato R, Kaga C, Kunimatsu M, Kobayashi T, Honda H. Peptide array-basedinteraction assay of solid-bound peptides and anchorage-dependant cells and itseffectiveness in cell-adhesive peptide design. J Biosci Bioeng. 2006, 101(6), 485-95.