1. HOME
  2. Peptide array-based screening method for medical device surface functionalization

Peptide array-based screening method for medical device surface functionalization

  • Graduate School of Pharmaceutical Sciences
  • Department of Basic Medicinal Sciences
  • Laboratory of Cell and Molecular Bioengineering
  • Division of Biosciences

Kei Kanie [Assistant Professor]

http://www.ps.nagoya-u.ac.jp/lab_pages/CMB/index-e.html

  • Graduate School of Pharmaceutical Sciences
  • Department of Basic Medicinal Sciences
  • Laboratory of Cell and Molecular Bioengineering
  • Division of Biosciences

Ryuji Kato [Associate Professor]

Outline of Seeds

For medical devices that are implanted for long time in vivo, their surface functionalization to proper interact with various types of cells is important for reducing side effects, and also important for enhancing their in vivo regeneration. Our seed technology is the peptide array-based efficient screening method for peptides that can functionalize the medical device surfaces. We can screen peptides for cell adhesion, cell differentiation, or objective protein pull down. Practically this is the technology of designing efficient diversity of peptide library by information analysis of peptide structural information, and actual direct assay with cells and proteins.

Novelty and originality of this research

In most of the cases which try to explore functional peptides for medical devices, the sequence is searched from partial protein molecules. However, since they are not screened on the "solid-bound form", the final candidate do not promise to function as expected after final product design and coating on material surface. However, our technology, which directly screen the peptide function on "solid-bound form peptide array", the screened results are feasibly reproduced on the product surfaces. Moreover, since the combinatorial variation of peptides are too wide to explore simply, we here introduce information analysis to select "independent" sequences efficiently on the limited surface area of array, and can enhance the screening efficiency.

Application and research area for Industry collaboration

We have been applying this technology for various applications;
- Screening of cell-selective peptides
- Screening of ECM-selective peptides
- Screening of differentiation enhancing peptides We are welcome to make collaborations with companies that produce medical devices, or produce medical polymers.

Key Takeaway

We are the only group in the world which can provide peptide array-based direct cellular assay, and efficient peptide library design using informatics.

Keywords

Medical devices, Cell adhesion, Cell selectivity, Peptides, Peptide screening, Peptide coating

Technologies

  • Cell-selective peptide sequences
  • Peptide array-based peptide screening method
  • Cell-selective peptide coating know-how

Monographs, Papers and Articles

  • Kanie K, Narita Y, Zhao Y, Kuwabara F, Satake M, Honda S, Kaneko H, YoshiokaT, Okochi M, Honda H, Kato R. Collagen type IV-specific tripeptides for selectiveadhesion of endothelial and smooth muscle cells. Biotechnol Bioeng. 2012, 109(7), 1808-16
  • Kanie K, Kato R, Zhao Y, Narita Y, Okochi M, Honda H. Amino acid sequencepreferences to control cell-specific organization of endothelial cells, smoothmuscle cells, and fibroblasts. J Pept Sci. 2011, 17(6), 479-86.
  • Kato R, Kaga C, Kunimatsu M, Kobayashi T, Honda H. Peptide array-basedinteraction assay of solid-bound peptides and anchorage-dependant cells and itseffectiveness in cell-adhesive peptide design. J Biosci Bioeng. 2006, 101(6), 485-95.